Physical and toxicological profiles of human IAPP amyloids and plaques
Publications | December 4, 2018

Dr Aleksandr Kakinen has published a paper on the physical and biological characteristics of human islet amyloid polypeptide (IAPP) fibrils. The paper was published in Elsevier’s Science Bulletin and is titledĀ Physical and toxicological profiles of human IAPP amyloids and plaques. The paper was co-authored by CBNS members Ibrahim Javed, Ava Faridi, Emily Pilkington, Dr Pu Chun Ke and CBNS Director Professor Tom Davis.

 

Abstract

Although much has been learned about the fibrillization kinetics, structure and toxicity of amyloid proteins, the properties of amyloid fibrils beyond the saturation phase are often perceived as chemically and biologically inert, despite evidence suggesting otherwise. To fill this knowledge gap, we examined the physical and biological characteristics of human islet amyloid polypeptide (IAPP) fibrils that were aged up to two months. Not only did aging decrease the toxicity of IAPP fibrils, but the fibrils also sequestered fresh IAPP and suppressed their toxicity in an embryonic zebrafish model. The mechanical properties of IAPP fibrils in different aging stages were probed by atomic force microscopy and sonication, which displayed comparable stiffness but age-dependent fragmentation, followed by self-assembly of such fragments into the largest lamellar amyloid structures reported to date. The dynamic structural and toxicity profiles of amyloid fibrils and plaques suggest that they play active, long-term roles in cell degeneration and may be a therapeutic target for amyloid diseases.